The protein is completely transferred from the gel to the membrane. The mixing liquid must be mixed well, and the aggregation time should be enough. Fluorescence detection keeps all items clean, ensuring that there is no background interference; the blotting membrane is kept in the dark; the background quenching plate is used to reduce background fluorescence and enhance the signal-to-noise ratio. Fluorescence method Western blot, washing the membrane can also remove the detergent with autofluorescence. You can choose a model that meets the needs of existing experiments.

NC membrane low molecular weight protein detection is usually used for chemiluminescence. What to prepare before Blot? When optimizing transfer settings, conditions and experiments, the most important thing is to determine whether all proteins in the sample are completely transferred from the gel to the membrane.

If the gel is not uniformly aggregated, it will cause poor glue resolution. Okay, then this editor-in-chief talks to you about those things in the western blot experiment process. It is usually used for fluorescence method (low autofluorescence background). When the protein is stripped and secondary hybridized, how is the laboratory manufacturers protein retention ability established? The common form of membrane transfer system for the membrane transfer system is the 'sandwich' structure. Set the threshold, detect the band 3.

Detection Azure Biosystems c-series imaging system Azure c-series imaging system provides 4 excellent Western blot imaging systems.Prospective review of protein separation, hybridization, detection, and analysis. View the results, you can make changes or edit any band boundary 5. I believe you have chosen your western blot experimental methods. If there are multiple sheets when washing the membrane, it is recommended to clean them separately. When electrophoresis of smiley frowning bands occurs, it is best to keep the sample volume and concentration consistent. How to choose the membrane? There are protein binding sites on the membrane that can bind proteins transferred from the gel


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